DNA encoded library (DEL) technology has permitted substantial leaps in compound screening by enabling an easier way to interrogate libraries and develop lead compounds. While a huge benefit to DEL technology is its reduction in the sheer number and tracking of discrete compounds through the screening process, it does come with a limitation in binding properties that can be gleaned for potential hits. In practice, from an initial screen numbering millions, the resulting thousands of hits are then reduced to around 50 compounds carried forward into downstream assays with limited characterization of their binding properties.

HT-SPR DEL assay metrics

Max throughput (detailed kinetic affinity) – 1,152 DELs/day, 5,760 DELs/week

DEL quantity: 44 pmol each

Target quantity (e.g., 20 kDa protein @ 6uM max conc) – 1,152 DELs: 165 ug, 5,760 DELs: 495 ug

Additional assay configurations available

Representative DEL kinetic screening data

  • 1:1 fit model lines in red
  • Automatic data flagging in analysis software highlighting low activity (gray) and complex binders (purple)
  • 1,152 affinities process in < 10 min

Example of data quality at multiple densities

Case study: kinetic uniformity

  • 6 DEL compounds tested as 8 replicates each
  • 47 kDa protein target injected from 1000 to 0.24 nM

≤ 10% variation across all on and off-rates