Epitope Mapping at the Amino Acid Level.
Label-Free Screening of mAb Libraries against a 384 Peptide Array.
SPRi can be used for antibody epitope discovery at the amino acid level by immobilizing a panel of up to 384 biotinylated peptides with overlapping sequences onto the SPR chip. A library of mAbs is then injected across the surface. The results can be processed with the Binning Software Tool to generate a heatmap and sort it by like behaved antibodies.
Alternatively, enzymatic digestion can be used to generate different sequence contexts for epitope discovery, which can be immobilized on the array via the CFM and probed with antibody libraries or patient sera.