Visit us at Booth #300 at the Antibody Engineering & Therapeutics conference in San Diego, CA.
Accelerating the Discovery of Therapeutic Antibodies Using High Throughput SPR
Presenter: Yasmina Noubia Abdiche, PhD, Chief Scientific Officer, Carterra
Throughput, speed, resolution, and sample consumption are typically key limiting- factors for detailed kinetic characterization early in antibody discovery campaigns. Here, we show that high throughput surface plasmon resonance (SPR) can be used to rapidly generate high quality kinetic data from 384 antibodies in parallel with minimal sample consumption. Additionally, epitope binning assays can be performed routinely on up to 384 antibodies per array, providing unprecedented throughput that allows for early assessment of your library’s epitope coverage with exquisite epitope discrimination, facilitating the identification of clones targeting unique epitopes. The ability to characterize binding kinetics, affinity, and epitope specificity on large antibody panels with minimal sample consumption at early stage research is highly advantageous in drug discovery because it helps to accelerate library-to-lead triage.
Implementing High-Throughput SPR in Recombinant Antibody Discovery and Library Development
Presenter: Sara D’Angelo, PhD, VP of Library Development, Specifica, Inc.
Date: Tuesday, December 10, 2019
Time: 12:15 pm – 1:15 pm
Location: San Diego Ballroom A
SPR-based technologies are catching up with the throughput required for the rapidly expanding field of antibody therapeutics, so becoming an invaluable support for many aspects of recombinant antibody development. This presentation will offer an overview of the kinetic analyses and epitope binning experiments that are routinely implemented at Specifica using the Carterra LSA in affinity maturation campaigns, lead prioritization from selection campaigns, and characterization of our next generation antibody libraries.