Reveal the Epitope Diversity of Your Antibody Library Using High Throughput SPR: A Case Study Using Progranulin

Key Takeaways

• Carterra’s LSA facilitates pairwise competition or epitope binning assays on hundreds of monoclonal antibodies (mAbs) in parallel to reveal the epitope landscape of your antibody library.
• Knowing your epitope coverage is key to the selection of mAbs with unique mechanisms of action and to securing intellectual property.
• Binning (competition assays) and mapping (screening against antigen variants, in this case, chimeras) can be performed in the same assay, streamlining epitope characterization and providing complementary information.
• Sample consumption is exceptionally low; typically, only 5 μg per mAb is required to generate a full 384 x 384 competition matrix, and doesn’t scale with the size of the antibody panel, providing an extremely efficient way of using precious material.
• Merging binning data with orthogonal data, such as mapping, library source, and cross-reaction to orthologs or homologs of the target antigen, provides a more comprehensive analysis of your antibody library, which facilitates the identification of clones with uniquely desirable characteristics.

Introduction

High throughput epitope binning provides an efficient method for interrogating the epitope diversity of a panel of monoclonal antibodies (mAbs). Typically, binning experiments are limited in scope and complexity due to the geometric way the assays scale on traditional interaction analysis platforms. For example, a 10 x 10 set of antibodies requires 100 unique tests to complete a bi-directional competition map, whereas a 96 x 96 set would require nearly 10,000 individual analyses.