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The proprietary Carterra LSAXT HT-SPR technology platform expands beyond antibody discovery to enable PROTAC®s, protein-protein inhibitors, kinase, and cytokine screening and characterization. The LSAXT delivers up to 100x the data in 10% of the time and with 1% of the sample requirements of other systems and consumable costs of other label-free instruments.
The LSAXT enables the following automated workflows in single unattended runs:
Selection of the appropriate sensor chip surface is essential for robust data quality when designing a high-throughput SPR assay. Carterra offers a broad range of chip surfaces to support the diverse applications available on the LSAXT.
The LSAXT expands the boundaries of HT-SPR with enhanced sensitivity and data quality.
When the assays become demanding, LSAXT provides all the benefits of the LSA plus:
Kinetic and specificity profiles for staurosporine (466.54 Da) binding an array of kinases in duplicate.
The LSAXT’s throughput, flexibility, and applications are enabled by three core system modules:
The Carterra LSAXT is covered by one or more of the following U.S. Patents: 8,210,119; 8,211,382; 8,383,059; 8,488,120; 8,999,726; 9,682,372; 9,682,396; 10,300,450
Cartridge consisting of a 96 array for:
Single Flow Cell:
Cartridge covers the entire chip surface for:
The 96-channel printhead enables a 96 protein array to be immobilized in parallel from flow onto a sensor chip surface in a single step. This can be repeated up to 4 times to build a 384 array on the SPR surface.
The sample deck adds another layer of throughput and flexibility.
96 or 384-well plates can be used in the LSAXT sample deck with 96-channel access in 3 bays and single flow cell access in 2 bays. The sample block has the capacity for 3 x 50ml conical tubes, 5 x 15ml conical tubes, and 8 x 1.5ml tubes.
Automated switching between the 96-channel printhead and single flow cell cartridges.
Quickly and efficiently setup experiments using Navigator’s streamlined interface and purpose-built assay wizards. Fully automated experiments allow unparalleled throughput and real-time binding data richness. Queue up to six assays to maximize throughput for up to 7 days of unattended run time.
Hands-on time is further reduced via automated data analysis which enables rapid processing and initial curation of large experiments. Dedicated application-focused analysis software follows intuitive, simple-to-use workflows and powerful visualization tools.
Complete kinetic data processing and analysis can be automated, with referencing, zeroing, cropping, blank subtraction, and kinetic model fitting all executed as a single operation for up to 1152 samples per unattended run.
In the binning tool, data are linked across panels to facilitate review of sensorgrams, heat maps, networks, and a dendrogram.
The ability to merge orthogonal data and view networks by color options provides a powerful and intuitive way of assessing your antibody panel’s diversity, while keeping an epitope-centric view.
The network plots above provide an alternate view of the data, where chords represent the blocking relationships, envelopes inscribe the bins, and colors depict merged data.
A binning dendrogram, showing an adjustable custom cut-height (red line at 4.1) next to a community plot that is clustered by the custom cut height, which provides a less granular view than the four network plots above where the cut height was zero.