Carterra LSA is a versatile, fully integrated, high throughput monoclonal antibody (mAb) characterization platform that combines patented continuous flow microfluidics with array surface plasmon resonance (SPR) detection to deliver up to 100x the data in 10% of the time and with 1% of the sample requirements and consumable costs of other systems.
The LSA enables the following automated applications in single unattended runs:
Cartridge consisting of a 96 array for:
Cartridge covers the entire chip surface for:
Multi-channel (96) cartridge
The 96-channel printhead enable a 96 protein array to be immobilized in parallel from flow onto a sensor chip surface in a single step. This can be repeated up to 4 times to build a 384 array on the SPR surface.
The sample deck adds another layer of throughput and flexibility.
96 or 384-well plates can be used in the LSA sample deck with multi-channel access in 3 bays and single-channel access in 2 bays. The sample block has the capacity for 3 x 50ml conical tubes, 5 x 15ml conical tubes, and 8 x 1.5ml tubes.
Automated switching between the single-channel and multi-channel cartridges.
A streamlined user interface enables quick and efficient experimental setup. A diverse range of experiments can be quickly defined using intuitive features, minimizing hands on time prior to assay start.
Hands-on time is further reduced via automated data analysis which enables rapid processing and initial curation of large experiments. Dedicated application-focused analysis software follows intuitive, simple-to-use workflows and powerful visualization tools.
Complete kinetic data processing and analysis can be automated, with referencing, zeroing, cropping, blank subtraction, and kinetic model fitting all executed as a single operation for up to 1152 samples per unattended run.
In the binning tool, data are linked across panels to facilitate review of sensorgrams, heat maps, networks, and a dendrogram.
The ability to merge orthogonal data and view networks by color options provides a powerful and intuitive way of assessing your antibody panel’s diversity, while keeping an epitope-centric view.
The network plots above provide an alternate view of the data, where chords represent the blocking relationships, envelopes inscribe the bins, and colors depict merged data.
A binning dendrogram, showing an adjustable custom cut-height (red line at 4.1) next to a community plot that is clustered by the custom cut height, which provides a less granular view than the four network plots above where the cut height was zero.
Selection of the appropriate sensor chip surface is essential for robust data quality when designing an Array SPR assay. Carterra offers a broad range of chip surfaces to support the diverse applications available on the LSA. Described here are the standard chip offerings for the LSA. Custom surfaces may be available upon request. Always consult with a Carterra Applications Scientist when selecting sensor chips.
|HC30M||Polycarboxylate hydrogel, medium charge density, 30nm coating thickness||Moderate ligand capacity and excellent diffusion characteristics|
|HC200M||Polycarboxylate hydrogel, medium charge density, 200nm coating thickness||Higher ligand capacity for increased levels of immobilization|
|CMDP||2D planar carboxymethyldextran surface, <5nm coating thickness||Lower ligand capacity and excellent diffusion characteristics|
|CMD50M||Carboxymethyldextran hydrogel, 50nm coating thickness||Moderate ligand capacity and improved diffusion characteristics|
|CMD200M||Carboxymethyldextran hydrogel, 200nm coating thickness||Higher ligand capacity for increased levels of immobilization|
|SAD200M||Streptavidin, immobilized in a carboxymethyldextran hydrogel, 200nm coating thickness||High capacity for biotinylated ligand applications|
|HCX30M||NHS activated polycarboxylate hydrogel, medium charge density, 30nm coating thickness||Preactivated HC30M, does not require activation step|
|HCX200M||NHS activated polycarboxylate hydrogel, medium charge density, 200nm coating thickness||Preactivated HC200M, does not require activation step|
While CMD is a well described chemistry for use in biosensors, the linear polycarboxylate chemistry of HC and HCX chips provides an alternate surface chemistry to that of CMD in instances of non-specific binding or when differential diffusion characteristics are warranted.
25mM MES pH 5.5, 0.01% Tween-20 is the recommended running buffer for HCX chips in order to extend half-life of the sensor chip surface during coupling of proteins.
CMD and HC/HCX sensor chips can be used to build custom capture surfaces such as anti-mouse or Protein A/G via amine coupling of capture molecules.
When stored at -20 °C CMD and HC/HCX surfaces are good for two years after purchase, while protein derivatized coatings such as SAD should be used within one year.