Epitope Mapping at the Amino Acid Level

Label-free screening of mAb libraries against a 384 peptide array.

The LSA can be used for antibody epitope discovery at the amino acid level by immobilizing a panel of up to 384 biotinylated peptides with overlapping sequences onto the SPR chip. A library of mAbs is then injected across the surface. The results can be processed with the Epitope Data Analysis Software to generate heatmap, dendrogram and stacked plot data views to aid in selection.

Alternatively, enzymatic digestion can be used to generate different sequence contexts for epitope discovery, which can be immobilized on the array via the CFM and probed with antibody libraries or patient sera.

Snapshot of the results of epitope mapping on a 384-peptide array showing sensorgrams, heat map, dendrogram, and stacked plot analyses.

Sorted heat map showing how the mAbs cluster into different epitope groups based on their peptide specificities.