Linear polycarboxylate
Name Description Application
HC30M Polycarboxylate hydrogel, medium charge density, 30nm coating thickness Moderate ligand capacity for general use
HC200M Polycarboxylate hydrogel, medium charge density, 200nm coating thickness Higher ligand capacity for general use

Carboxymethylated dextran
Name Description Application
CMDP 2D planar carboxymethyldextran surface, <5nm coating thickness Lower ligand capacity for high resolution analysis
CMD50M Carboxymethyldextran hydrogel, medium charge density, 50nm coating thickness Moderate ligand capacity for general use
CMD200M Carboxymethyldextran hydrogel, medium charge density, 200nm coating thickness Higher ligand capacity for increased levels of immobilization
CMD500M Carboxymethyldextran hydrogel, medium charge density, 500nm coating thickness Higher ligand capacity for maximum levels of immobilization

NiNTA
Name Description Application
NiHC200M Poly – NTA derivatized linear polycarboxylate hydrogel, medium charge density, 200nm coating thickness High capacity for His-tagged ligand captures

Streptavidin
Name Description Application
SAP Streptavidin immobilized on 2D planar carboxymethyl dextran surface, <5nm coating thickness Very low capacity for arraying of biotinylated ligands
SAHC30M Streptavidin, immobilized in polycarboxylate hydrogel, medium charge density,
30nm coating thickness

Lower capacity for biotinylated ligand kinetic lawns

SAD200M

Streptavidin, immobilized in a carboxymethyldextran hydrogel, medium charge density, 200nm coating thickness

High capacity for biotinylated ligand captures


Protein A
Name Description Application
PAHC200M Protein A derivatized linear polycarboxylate hydrogel, medium charge density, 200nm coating thickness

High capacity for quantitation of human IgG


Protein A/G
Name Description Application
PAGHC30M Protein A/G derivatized linear polycarboxylate hydrogel, medium charge density, 30nm coating thickness

Moderate capacity for kinetics of multi-species IgG

PAGHC200M Protein A/G derivatized linear polycarboxylate hydrogel, medium charge density, 200nm coating thickness High capacity for quantitation of multi-species IgG

 

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Notes

While CMD is a well described chemistry for use in biosensors, the linear polycarboxylate chemistry of HC chips provides an alternate surface chemistry to that of CMD in instances of non-specific binding or when differential diffusion characteristics are warranted.

CMD and HC sensor chips can be used to build custom capture surfaces such as anti-mouse or Protein A/G via amine coupling of capture molecules.

When stored at -20 °C CMD and HC surfaces are good for two years after purchase, while protein derivatized coatings such as SAD should be used within one year.

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