Visit us at Booth #10 at the Antibody Engineering & Therapeutics conference in Amsterdam, Netherlands.
Scientific Briefing Presentation
High Throughput SPR Demonstrates that V-lambda Expressing OmniChickens™ Exhibit Broad Epitope Coverage and Picomolar Affinity
Presenter: Kathryn Ching, PhD, Senior Scientist, Ligand Pharmaceuticals
Date: Wednesday, June 12
Time: 1:30 pm – 2:00 pm
Location: Room 12 – 14 (upstairs)
The generation of antibodies to therapeutic targets with high conservation between rodents and humans is a challenge. Because of their increased evolutionary distance from humans compared to rodents, chickens are an ideal species to circumvent this challenge. Here we introduce a new line of OmniChickens™ which express VL1-44. The use of high throughput SPR allowed us to tease out more subtle relationships between antibodies in a given epitope bin. The analysis of large cohorts of antibodies in a single experiment against a model antigen in the different OmniChicken lines is an important metric in assessing the immune repertoire of our birds.
Accelerating the Discovery of Therapeutic Antibodies Using High Throughput SPR
Presenter: Andrew Goodhead, PhD, Carterra
Poster Board Number: H6
Throughput, speed, resolution, and sample consumption are typically key limiting- factors for detailed kinetic characterization early in antibody discovery campaigns. Here, we show that high throughput surface plasmon resonance (SPR) can be used to rapidly generate high quality kinetic data from 384 antibodies in parallel with minimal sample consumption. Additionally, epitope binning assays can be performed routinely on up to 384 antibodies per array, providing unprecedented throughput that allows for early assessment of your library’s epitope coverage with exquisite epitope discrimination, facilitating the identification of clones targeting unique epitopes. The ability to characterize binding kinetics, affinity, and epitope specificity on large antibody panels with minimal sample consumption at early stage research is highly advantageous in drug discovery because it helps to accelerate library-to-lead triage.