Visit us at Booth #308 at PepTalk in San Diego, CA.
Accelerate Kinetic Screening and Epitope Characterization of Antibody Libraries with Array SPR
Presenter: Yasmina Noubia Abdiche, PhD, Chief Scientific Officer, Carterra
Dates and Times: Monday, January 14 from 6:00pm – 7:15pm; Tuesday, January 15 from 9:50am – 11:00am and 1:30pm – 2:00pm
Throughput, speed, resolution, and sample consumption are typically key limiting factors for performing detailed kinetic and epitope characterization of monoclonal antibody (mAb) libraries destined for use as therapeutics. In addition, mAb immune responses can be used to survey the epitope landscape of pathogens and inform the design of better immunogens for vaccines. In this poster, we demonstrate three core applications of Array SPR: capture kinetics, epitope binning, and epitope mapping. These applications provide a comprehensive characterization of your mAb library with minimal sample consumption, enabling more confident decisions to be made earlier in the research process and removing the need for preliminary ELISA screening. We show that Array SPR can facilitate the generation of high quality kinetic data from 384 mAbs in parallel, using <1 µg per mAb and only 2 µg of antigen. Epitope binning in a 384-mAb array format allows for a rapid assessment of the depth and breadth of your library’s epitope diversity, providing exquisite resolution between near-identical clones, using <5 µg per mAb and about 100 µg antigen. Finally, a large panel of mAbs can be mapped against a 384-peptide library, if their epitopes can be (partially) recapitulated on peptides, using 1µg per mAb and <0.1 µg per peptide.