Visit us at Booth #1609 at SLAS in Washington, DC.
Biologics Discovery Podium Presentation
Accelerate Library-to-Lead Triage of Antibody Libraries Using Array SPR
Presenter: Yasmina Noubia Abdiche, PhD, Chief Scientific Officer, Carterra
Date: Monday, February 4
Time: 3:00pm – 3:30pm
Throughput, speed, resolution, and sample consumption are often key limiting factors for performing detailed kinetic and epitope characterization of antibody libraries destined for use as therapeutics or supporting diagnostics and reagents. In addition, antibody immune responses can be used to survey the epitope landscape of pathogens and inform the design of better immunogens for developing vaccines. Here, we demonstrate three core applications of Array SPR that can be performed routinely and quickly in a 384-array format; (1) capture kinetics, (2) epitope binning, and (3) epitope mapping. Together, these assays provide a comprehensive characterization of your antibody library with minimal sample consumption, often less than 5 micrograms per antibody for most applications, enabling more confident decisions to be made earlier in the research process and obviating the need for preliminary ELISA screening. We show that Array SPR can generate high quality capture kinetic data from 384 mAbs in parallel using less than 10 micrograms of purified antigen, making it highly efficient on antigen consumption. Epitope binning in a 384-array format allows for a rapid “high-definition” assessment of the depth and breadth of your library’s epitope diversity, providing exquisite resolution between near-identical clones, which can aid in the identification of new epitopes and shed light on mechanism of action. Since antibody consumption does not scale with the size of the panel being investigated when using Array SPR, a 384×384 binning matrix can be accomplished with the same antibody sample consumption as for a smaller panel. Finally, arraying a peptide library facilitates the epitope mapping of a large panel of antibodies (384+), if their epitopes can be recapitulated on peptides.