Festival of Biologics 2023 – San Diego

Speaker session

Title: The new LSA^XT and high-throughput SPR characterization of multi-specific antibodies

Speaker: Daniel Bedinger, PhD, Carterra

Date: Monday, Mar. 20, 2023

Time: 3:20–3:40 pm PT

Location: Driftwood 2

Abstract: The Carterra LSA platform is the leading platform for high-throughput kinetics and epitope analysis (HT-SPR technology) of mAbs and protein therapeutics. The new LSA^XT platform has enhanced sensitivity, further extending the application space of HT-SPR to include molecule classes such as kinase inhibitors, PROTAC®S, and transient interactions like Fc Receptors.  Selection of bi and tri-specific binders often requires the screening of large combinatorial sample sets derived from panels of monospecific binders.  The Carterra LSA and LSA^XT make analyzing the binding properties of these combinatorial panels straightforward and requires minimal amounts of mAb sample and antigen. The affinity of the binders to the targets can be measured in several assay formats and used to verify the independence and activity of each binding site for hundreds of clones. Along with the binding kinetics and specificity measurements, the LSA and LSA^XT enable large scale epitope binning to ensure diverse sets of clones are being carried forward to functional evaluation.