Conference dates: MARCH 9-11, 2022
Location: Marriott Marquis San Diego
Carterra Booth #17
Speaker: Tom Z. Yuan, PhD
Title: Leveraging synthetic biology to rapidly characterize neutralizing antibodies to Ebolavirus and SARS-CoV-2 from convalescent donors and synthetic antibody libraries.
Date: Thursday March 10, 2022
Time: 5:20 PST
Location: Room 14
Abstract: Development of neutralizing antibodies is dependent upon broad epitope coverage to increase the likelihood of achieving therapeutic function. Advances in synthetic biology have allowed us to conduct an epitope binning study on a large panel of antibodies identified to bind to Ebola virus glycoprotein with only published sequences. Additionally, we describe the discovery and characterization of neutralizing SARS-CoV-2 antibodies from large-scale phage libraries, each constructed synthetically utilizing shuffled CDR loops from natural human and llama antibody repertoires. Several neutralizing antibodies target rare epitopes that resists mutagenic escape by SARS-CoV-2 variants of concern. Synthetic biology advances allow us to rapidly identify and characterize antibodies with therapeutically desirable features, including high affinity, unique binding sites, potent neutralizing activity in vitro, and a capacity to limit disease in vivo animal studies.
Title: Improving the screening, selection and deep characterization of biotherapeutic molecules using high-throughput SPR
Abstract: Throughput, speed, resolution, and sample consumption are inevitable challenges scientists face when screening biomolecule libraries and working to the goals of discovering and identifying the best lead candidates. Educated selection will often require multiple tests and orthogonal testing confirmation to determine the top candidate. Here we demonstrate how the Carterra LSA, which performs high-throughput surface plasmon resonance (HT-SPRTM) can rapidly generate high quality kinetic data from 384 candidates in parallel using a minimal amount of sample material. In addition, the LSA can perform epitope binning on up to 384 candidates per array to obtain unique epitope discrimination and characterization. The LSA is a high-throughput biology solution that enables scientists to characterize binding kinetics, affinity, and epitope specificity on large protein panels with minimal sample consumption, accelerating library-to-lead candidate selection and discovery.
Come to the Carterra booth and if you take a short 7 question survey, you can take home a Carterra scientist plush toy bear.