Oxford Global: NextGen Biomed 2025
Conference dates: March 12-14, 2025
Stand: 6
Location: London, UK
Speaking session
Title: Use of Carterra Platforms for High Throughput SPR for Applications Including Kinetic Parameter Measurement of Fabs in PPE to Fragment Screening in High RI Solvents
Presenter: Jon Popplewell , PhD, Field Applications Scientist, Carterra
Date and Time: Thursday, March 13, 2025, 8:45 – 8:55 AM GMT
Location: Innovation & Collaboration – Conference Rm 10, Talbot Suite
Abstract: Carterra will introduce its three SPR platforms which cover a full range of SPR applications. The LSA and LSAXT are primarily designed at measuring kinetic parameters of biological samples, including measurement of the binding kinetics of Fab candidates directly from crude PPE as described in the accompanying poster, whilst the newest platform, the Ultra, is focused on the small molecule and fragment screening area of SPR applications. This talk will present data from all the 3 platforms, showcasing the diverse applications the Carterra platforms can deliver.
Poster session
Poster Title: Full Antigen Kinetics of Fabs Directly from Crude Periplasmic Extracts on an Anti-CH1 Capture Surface
Presenter: Jonathan Popplewell, PhD, Field Applications Scientist, Carterra
Abstract: Characterizing detailed binding kinetics of antibody fragments directly from bacterial periplasmic extracts (PPEs) has been historically challenging because purification of all primary hits is time and resource intensive. Here we have described a highly scalable, yet data rich approach to characterizing the binding kinetics of Fab candidates directly from crude PPE sources. The throughput of the LSAXT can measure full kinetics (ka, kd, and KD) for thousands of Fabs in a single week, while eliminating the need for purification. The assay demonstrated here using 96 unique Fabs required fewer than 9 ug of antigen and was completed in 10 hrs. Importantly, this set of 96 clones is only one-twelfth of the 1152 clone capacity that can be analyzed in a single experiment. The ability to quantitate complete kinetic profiles for responses with Rmax values as low as 5 RU emphasizes that data can be obtained even for poorly expressing clones and is a key benefit of the enhanced sensitivity of the LSAXT.