Conference dates: NOVEMBER 14-16, 2022

Stand: 11

Speaking session

Title: Integrating The Carterra LSA High-Throughput SPR Array in to UCB’s Antibody Discovery Platform

Speaker: Oliver Zaccheo, PhD

Speaker Title: Senior Group Leader, SPR and Biophysical Assays, UCB

Date: Tuesday, November 15, 2022

Time: 13:20 CEST

Abstract: Surface Plasmon Resonance (SPR) provides a key screen to identify and characterize high-affinity, species cross-reactive antibodies generated by UCB’s Core antibody discovery platform.  We have performed a head-to-head comparison of the Biacore 4000 and Carterra’s LSA (high-throughput SPR array) and shown that the LSA is well-suited to the challenge of generating high-quality, high-throughput antibody binding data from individual b-cells.  We have now incorporated the LSA to our routine workflows to guide molecule selection based on kinetic binding data and epitope binning.

Poster session

Title: Improving the screening, selection and deep characterization of biotherapeutic molecules using high-throughput SPR

Abstract: Throughput, speed, resolution, and sample consumption are inevitable challenges scientists face when screening biomolecule libraries and working to the goals of discovering and identifying the best lead candidates. Educated selection will often require multiple tests and orthogonal testing confirmation to determine the top candidate. Here we demonstrate how the Carterra LSA, which performs high-throughput surface plasmon resonance (HT-SPR) can rapidly generate high quality kinetic data from 384 candidates in parallel using a minimal amount of sample material. In addition, the LSA can perform epitope binning on up to 384 candidates per array to obtain unique epitope discrimination and characterization. The LSA is a high-throughput biology solution that enables scientists to characterize binding kinetics, affinity, and epitope specificity on large protein panels with minimal sample consumption, accelerating library-to-lead candidate selection and discovery.