Characterizing Challenging Membrane Targets Using HT-SPR

Membrane targets make up a substantial part of the overall “undruggable” therapeutic space that has recently garnered widespread interest. Despite encouraging improvements in the tools to screen for therapeutics against membrane-bound targets, there are still many practical limitations owing to the challenges of working with proteins that are not highly stable outside of the cell-membrane environment.

In conjunction with ACROBiosystems, we have found that High-throughput surface plasmon resonance (HT-SPR) is a powerful technique that is transforming characterization workflows and enabling a greater breadth and depth of information for up to thousands of drug candidates.

In this poster, we demonstrate:

• The ability to quantitatively assess binding kinetics for panels of antibodies against membrane receptors in several formats.
• How this workflow highlights opportunities to perform detailed binding characterization for up to thousands of drug candidates in parallel.
• How we can develop detailed kinetic profiles for challenging membrane targets