Presentation: High-throughput biophysical characterization of monoclonal antibodies for identifying epitope diversity at NGM Biopharmaceuticals

Presented by: Zimple Matharu, PhD, Principal Scientist, NGM Biopharmaceuticals

Abstract: Successful discovery of therapeutic antibodies with desired biological function centers around finding a suitable antibody candidate with epitope that influences the function of the target protein. By prioritizing affinity-based selections over epitope-based selections important functional epitopes could go unidentified. With the help of array-SPR Carterra platform high-throughput epitope binning analysis on a large mAb panel could be performed in the early drug discovery stage. The combination of epitope binning results with kinetics and sequence analysis can provide an efficient way of selecting high affinity antibodies representing a diverse set of sequence families and epitopes. These studies may further provide an effective way of identifying domain specific antibody binders. The detailed analysis would be discussed at the upcoming symposium.