Rapid Screening and Kinetic Analysis of Small Molecules Using Carterra Vega™—a 48-Channel HT-SPR Platform

Highlights

• Carterra Vega™ is a high-throughput SPR platform that provides 48-analyte parallel analysis through its multi-channel flow cell, facilitating high-quality binding data collection for up to two targets and an internal reference per channel.
• Vega screened a 384-well plate in 35 minutes in this application, enabling rapid hit identification with kinetic context. With fast cycle times and an optional integrated plate-loading robot, Vega can screen greater than 20,000 compounds per day.
• Vega accelerates hit confirmation by streamlining full kinetic characterization through titration.
• Purpose-built for modern drug discovery, Vega reduces rework between screening and characterization to accelerate hit-to-lead decisions.

Introduction

Modern drug discovery relies on two key capabilities: 1) high-throughput experimental screening of large, diverse chemical or biological libraries, and 2) a detailed understanding of molecular interactions to enable rational design and optimization. However, these two needs are often seen as a trade-off in early discovery, with campaigns typically emphasizing speed and broad coverage in primary screening, deferring mechanistic and selectivity characterization until later, when time, material requirements, and downstream risks increase. To bridge this gap, there is a growing need for platforms that combine screening-scale throughput with high-quality characterization.