Throughput, speed, resolution, and sample consumption are typically key limiting factors for detailed kinetic characterization early in monoclonal antibody (mAb) discovery campaigns. Here, we show that high-throughput SPR (HT-SPR) can facilitate the generation of high-quality kinetic data from a large panel of clones rapidly and with minimal sample consumption. In this example of a single day’s run, 384 independent kinetic measurements were made on an array comprised of 43 unique mAbs, each immobilized at 8-16 capacities, using a capture approach which does not require purified antibodies. This method required <1 μg per mAb and only 2 μg of the recombinant monomeric antigen. The array format provided well-described and highly reproducible kinetic measurements for clones spanning a 10,000-fold affinity range for their target antigen. These data clearly demonstrate the efficiency and quality of kinetic analysis that is possible using HT-SPR.