Conference dates: FEBRUARY 5-9, 2022
Carterra Booth #1246
Speaker: Noah T. Ditto, MS, MBA & Jennifer Carlstrom, PhD
Title: Improving therapeutic discovery by leveraging HT-SPR to complement AlphaLISA
Date: Wednesday, Feb. 9, 2022
Time: 12:30pm – 12:50pm EST
Location: Exhibition Theater (#2644 in Exhibition)
Abstract: Therapeutic discovery and development rely on numerous analytical techniques in order to best position candidate drugs for commercial success. Different analytical technologies are utilized for their respective strengths in maximizing understanding of candidate properties. Reliable FcRn binding assays are used in both discovery and development of therapeutic antibodies to predict the half-life in vivo. Here we show two orthogonal assays for measuring binding of antibodies to FcRn. The AlphaLISA FcRn binding assay is a robust high throughput no-wash immunoassay that was used to measure relative affinities of therapeutic antibodies to FcRn. We then describe the power of HT-SPR as an orthogonal approach to reinforce AlphaLISA potency findings. The real-time, multiplexed, and label-free nature of HT-SPR enables a diverse range of measures to be obtained from a relatively simple set of experiments. Collectively, these studies highlight how AlphaLISA and HT-SPR can be used in conjunction to facilitate a more thoughtful understanding of therapeutic candidate attributes.
Title: Improving the screening, selection and deep characterization of biotherapeutic molecules using high-throughput SPR
Date: Tuesday Feb. 8, 2022
Time: 2:30 pm – 3:30 pm EST
Presenter: Noah T. Ditto
Abstract: Throughput, speed, resolution, and sample consumption are inevitable challenges scientists face when screening biomolecule libraries and working to the goals of discovering and identifying the best lead candidates. Educated selection will often require multiple tests and orthogonal testing confirmation to determine the top candidate. Here we demonstrate how the Carterra LSA, which performs high-throughput surface plasmon resonance (HT-SPRTM) can rapidly generate high quality kinetic data from 384 candidates in parallel using a minimal amount of sample material. In addition, the LSA can perform epitope binning on up to 384 candidates per array to obtain unique epitope discrimination and characterization. The LSA is a high-throughput biology solution that enables scientists to characterize binding kinetics, affinity, and epitope specificity on large protein panels with minimal sample consumption, accelerating library-to-lead candidate selection and discovery.
Come to the Carterra booth and if you take a short 7 question survey, you can take home a Carterra scientist plush toy bear.