Greetings, Carterra Users! We hope that everyone is having a wonderful year. The Support Team at Carterra is very excited to keep you updated on all of the advancements in high-throughput SPR and updates to our platform. Please see our summer update below.
The LSAXT is now available for purchase as well as an upgrade to your existing LSA! There are many amazing features included with our new product, including:
- 2-fold better signal-to-noise
- Faster data collection rate
- Less than 15% signal variability across the array
For more information, please contact cgiddens@carterra-bio.com
Carterra has expanded our sensor chip line-up with additional offerings:
- PAGP
- NiCMDP
- RSA200M (regeneratable streptavidin)
New software versions have been released! Please update your software by logging into the Carterra web store and downloading the software. Release notes are also available highlighting new features and bug fixes.
Kinetics: https://carterra-bio.com/resources/kinetics-software/
Epitope: https://carterra-bio.com/resources/epitope-software/
If you have updated your software to the latest versions and are still experiencing slower than usual behavior or repeated crashing, check your PC settings to ensure that the performance setting is set to “Best Performance.” You can find this under System > Power & Battery > Power mode
(Windows 11)
(Windows 10)
A number of customers have reported a “Data Collection Error – No SPR Data Collected for 10 Seconds” that coincided with a Windows update push. If you encounter this error, please perform the following procedure: Powercycle PDF
Some of our customers have continued to encounter issues with EDC purity. EDC always contains some level of diamine contamination. When this level of contamination is high, it can inhibit chip capacity and protein preconcentration. HC chips, with their linear polycarboxylate, are more sensitive to these contaminates than CMD chips. As such, we recommend reducing the EDC concentration when performing surface prep lawn experiments from the typical final [C] of 133 mM to a new recommendation of ~ 75 mM final [EDC] or below, if preconcentration appears to be inhibited. This will dilute out the effect of the diamine contaminates and ensure the chip capacity remains as expected in addition to allowing normal preconcentration. Multichannel Activation concentrations of ~ 15 mM EDC, 5 mM S-NHS are not affected by this updated recommendation.
The LSA® and LSAXT both utilize SPR to measure real-time binding kinetics. As such, we track the minima of an SPR dip along a defined axis of possible locations and translate that as an RU signal. When the correct laser angle is chosen for a given refractive index solution, the raw data will be charted around 5-6K RU. This provides optimal SPR dip location within the detection range. When the data is charted below 3K or above 18K RU we risk a scenario where the SPR dips leave the detection range. This can result in straight line traces or swooping/hooking traces for any ROIs that had an SPR dip out of range. If you find your initial RAW charted data is below 3K or above 18K RU please abort the run and select a new refractive index (i.e., High, Med, Low for the LSAXT or HBS, MES, SAD for LSA) from the dropdown menu on the Experiment Runner page.
Genna Farner is our new point of contact for Price Quotes and Service Contracts. Any related questions can be directed to her email at gfarner@carterra-bio.com.
Have inquiries or requests related to PM Services? Please email service@carterra-bio.com.
The Support Team has curated a document that compiles technical tips, reminders, and guides for all things LSA. In this PDF, you will find advice for required maintenance, how to properly take care of your instrument, and how to prepare your experiments to achieve maximum success!
Feel free to print this PDF and stick it to the front of your LSA for optimal use and quick access.
If you are a new LSA user or a customer in Europe, you can reach out to the Support team at support_eu@carterra-bio.com to ensure that you receive prompt responses despite the time difference.
If you are a new user of the LSA, you can reach out to the Support team at support@carterra-bio.com and introduce yourself. We would love to hear from you and begin building a relationship to facilitate the learning process.