Greetings, Carterra Users! We hope that everyone is having a wonderful year. The Support Team at Carterra is very excited to keep you updated on all of the advancements in high-throughput SPR and updates to our platform. Please see our quarterly update below.
LSAXT upgrade is now available! There are many amazing features included with our new product, including:
- 2-fold better signal-to-noise
- Faster data collection rate
- Enhanced signal variability across the array
For more information, please contact cgiddens@carterra-bio.com
Carterra now has a new chip shipping process.
- In an effort to lessen our packaging waste, Carterra has adjusted our shipping procedure for some of our chip selections.
- CMD chips are thermally resilient and will be shipped at RT.
- HC chips are thermally resistant at RT for the duration of the shipping period (2 days) but should be placed into -20 freezer when received.
- NiHC and derivatized chips are not included in this change and will continue to be shipped with cold packs and should be stored at -20 once received.
Support remote assistance scheduling is easier than ever!
Click the hyperlink in the support staff’s email signature below to book time with a Technical Support Scientist.
Ira Seferovich
Applications Scientist & Technical Support Manager
Book time with Ira Seferovich: Technical Support Remote Session
Sophia Zuno
Technical Support Scientist
Book time with Sophia Zuno: Technical Support Remote Session
Some of our customers have continued to encounter issues with EDC purity. EDC always contains some level of diamine contamination. When this level of contamination is high, it can inhibit chip capacity and protein preconcentration. HC chips, with their linear polycarboxylate, are more sensitive to these contaminates than CMD chips. As such, we recommend reducing the EDC concentration when performing surface prep lawn experiments from the typical final [C] of 133 mM to a new recommendation of ~75 mM final [EDC] or below, if preconcentration appears to be inhibited. This will dilute the effect of the diamine contaminates and ensure the chip capacity remains as expected, in addition to allowing normal preconcentration. Multichannel Activation concentrations of ~15 mM EDC, 5 mM S-NHS are not affected by this updated recommendation.
We have multiple customers that transfer completed experiment files to remote server locations to complete the analysis. Please ensure that the file that will be analyzed is completely transferred to the local PC before attempting to open the file, otherwise the software will throw an error.
It has come to our attention that some of our customers are running biologics and systems that are more prone to growth and contamination than others. If you are running unpurified biological material, supernatants, or other solutions that can easily allow bacterial or fungal growth, our recommendation is to run a modified sanitization routine weekly or as needed. (Approximate time = 3 h.)
-
- Prime the SFC and PH on the cleaning slides twice in 0.1% NaClO
- Prime the SFC and PH on the cleaning slides twice in 3% Contrad
- Prime the SFC and PH on the cleaning slides three times in DI H2O + 0.05% T-20
We have been getting a few reports about PCs rebooting during experiment runs due to Windows Updates. To prevent this from happening, the user must pro-actively install updates or they will be at the mercy of Windows and however the program determines when to update and restart the PC.
This is especially important when using Pause Updates because there are circumstances where Windows will not honor a Pause request, particularly if too much time has passed or an update is urgent.
It is frequently mentioned that “I rebooted the computer, but it says I still need updates.” This is because unpausing updates and/or rebooting the PC does not, in itself, install anything. For updates to install automatically, the PC has to be idle for a period of time, which is rarely convenient. Because of this, the best-practice is to install updates and reboot before starting Navigator.
To bring the PC up-to-date (it’s best to do this before starting Navigator):
-
- Open Settings -> Update and Security -> Windows Updates (or type Windows Update in Search)
- Click “Check for Updates”
- Wait for it to complete the update check
- Download and install the listed updates
The Support Team has curated a document that compiles technical tips, reminders, and guides for all things LSA. In this PDF, you will find advice for required maintenance, how to properly take care of your instrument, and how to prepare your experiments to achieve maximum success. Feel free to print this PDF and attach it to the front of your LSA for optimal use and quick access.
If you are a customer in Europe, you can reach out to the European Support team at support_eu@carterra-bio.com to ensure that you receive prompt responses.
If you are a new user of the LSA, you can reach out to the Support team at support@carterra-bio.com and introduce yourself. We would love to hear from you to facilitate the learning process.