PEGS Europe 2026

Poster session

Poster 1 Title: Accelerating the Discovery of Therapeutic Antibodies Using High Throughput SPR

Abstract: Throughput, speed, resolution, and sample consumption are typically key limiting- factors for detailed kinetic characterization early in antibody discovery campaigns. Here, we show that high throughput surface plasmon resonance (SPR) can be used to rapidly generate high quality kinetic data from 384 antibodies in parallel with minimal sample consumption. Additionally, epitope binning assays can be performed routinely on up to 384 antibodies per array, providing unprecedented throughput that allows for early assessment of your library’s epitope coverage with exquisite epitope discrimination, facilitating the identification of clones targeting unique epitopes. The ability to characterize binding kinetics, affinity, and epitope specificity on large antibody panels with minimal sample consumption at early stage research is highly advantageous in drug discovery because it helps to accelerate library-to-lead triage.

Poster 2 Title: Strategies for Scalable and Sensitive Determination of High Affinity and Slow Dissociating Binding Interaction Kinetics in Antibody Discovery

Abstract: Monoclonal antibodies are powerful tools for therapeutic and diagnostic applications and binding kinetics is an important element of the potency and efficacy of these molecules. Many antibodies and optimized therapeutics show very high affinities with stable dissociation kinetics, and these high affinity interactions represent a challenge for accurate measurement using real time SPR binding studies, as slow dissociation yields minimal signal change over typical time courses. This poster explores two effective strategies using HT-SPR to measure slow dissociation of many interactions in parallel and with high sensitivity. The most sensitive and simple approach is the use of a chase injection to measure surface occupancy after a long dissociation period. The other is the use of both short and long dissociation times, which can enable an extended dissociation phase without excessively extending the overall length of an experiment. Both these approaches can be implemented at scale for the screening and characterization of hundreds to thousands of high affinity interactions using Carterra HT-SPR platforms.