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Carterra LSAXT for Drug Discovery & Development
Disruptive Technology for Screening and Characterization
Enhanced sensitivity and fast data collection rate build upon unmatched capabilities in throughput and enable assays such as TPDs/PROTAC®s, kinase, cytokines, DELs, and protein:protein inhibitors.
Carterra LSAXT Platform
The proprietary Carterra LSAXT HT-SPR technology platform expands beyond antibody discovery to enable PROTAC®s, protein-protein inhibitors, kinase, and cytokine screening and characterization. The LSAXT delivers up to 100x the data in 10% of the time and with 1% of the sample requirements of other systems and consumable costs of other label-free instruments.
Workflows
The LSAXT enables the following automated workflows in single unattended runs:
- Capture-based kinetics and affinity: Screen up to 1152 ligands
- Coupled kinetics: Up to 384 immobilized ligands
- Epitope binning: Interrogate up to 384×384 pairwise, competitive interactions
- Quantitation: Measure concentration of up to 1152 samples
- Peptide mapping: Screen up to 384 analytes against an array of up to 384 peptides
- Blocking / neutralization assays: 384 assay capacity
- Regeneration scouting: Scout 96 conditions in parallel
- General multiplexed applications: Array any combination of up to 384 ligands
Available LSAXT Biosensor Chip Chemistries
Carterra LSAXT Sensor Chips
Selection of the appropriate sensor chip surface is essential for robust data quality when designing a high-throughput SPR assay. Carterra offers a broad range of chip surfaces to support the diverse applications available on the LSAXT.
Expanding the Breadth of Applications
The LSAXT expands the boundaries of HT-SPR with enhanced sensitivity and data quality.
When the assays become demanding, LSAXT provides all the benefits of the LSA plus:
- Enhanced sensitivity
- Faster data collection rate
- Measure analytes down to 500 Da
Kinetic and specificity profiles for staurosporine (466.54 Da) binding an array of kinases in duplicate.
Unique Platform Technology
The LSAXT’s throughput, flexibility, and applications are enabled by three core system modules:
1. Patented flow cell technology 2. High throughput SPR 3. Software
The Carterra LSAXT is covered by one or more of the following U.S. Patents: 8,210,119; 8,211,382; 8,383,059; 8,488,120; 8,999,726; 9,682,372; 9,682,396; 10,300,450
1. Unique Patented Flow Cell Design & Sample Deck
The LSAXT has a unique patented flow cell design that provides both throughput and flexibility via two “modes” that switch automatically depending on the assay format:
96-Channel Printhead:
Cartridge consisting of a 96 array for:
- Continuous flow microspotting for discrete immobilization of up to 96 ligands in parallel
- Nest up to 4x 96 prints on a single chip surface for 384 interaction measurements in parallel
- ~200 μL per immobilization
Single Flow Cell:
Cartridge covers the entire chip surface for:
- One-on-many interactions
- Surface “lawn” preparations
- ~270 μL per interaction
96-channel printhead
The 96-channel printhead enables a 96 protein array to be immobilized in parallel from flow onto a sensor chip surface in a single step. This can be repeated up to 4 times to build a 384 array on the SPR surface.
The sample deck adds another layer of throughput and flexibility.
96 or 384-well plates can be used in the LSAXT sample deck with 96-channel access in 3 bays and single flow cell access in 2 bays. The sample block has the capacity for 3 x 50ml conical tubes, 5 x 15ml conical tubes, and 8 x 1.5ml tubes.
Automated switching between the 96-channel printhead and single flow cell cartridges.
2. High Throughput Surface Plasmon Resonance
A high-resolution CCD camera images the entire chip surface simultaneously, enabling up to 384 real-time interactions to be monitored in parallel with local referencing via neighboring interspots.
3. Industry-leading Experimental Setup, Data Analysis and Visualization Software
Navigator Software
Quickly and efficiently setup experiments using Navigator’s streamlined interface and purpose-built assay wizards. Fully automated experiments allow unparalleled throughput and real-time binding data richness. Queue up to six assays to maximize throughput for up to 7 days of unattended run time.
Key Benefits:
- Reduce hands-on time: Setup even the most complex assay in less than 5 minutes
- Adapt to any sample type: Predefined assay routines for purified or crude samples
- Simplify assay prep: Straightforward volume estimation for all assay buffers and reagents
- Multi-task and plan efficiently: Accurate experimental time estimation and in-run progress
- Walk-away convenience: Standby flow option keeps your surface ready to use between experiments
- Maximize data per experiment: Generate up to 150,000 interactions in a single, unattended run
Hands-on time is further reduced via automated data analysis which enables rapid processing and initial curation of large experiments. Dedicated application-focused analysis software follows intuitive, simple-to-use workflows and powerful visualization tools.
Kinetics Analysis Software
Complete kinetic data processing and analysis can be automated, with referencing, zeroing, cropping, blank subtraction, and kinetic model fitting all executed as a single operation for up to 1152 samples per unattended run.
Learn More about our Kinetics Software
Epitope Analysis Software
In the binning tool, data are linked across panels to facilitate review of sensorgrams, heat maps, networks, and a dendrogram.
The ability to merge orthogonal data and view networks by color options provides a powerful and intuitive way of assessing your antibody panel’s diversity, while keeping an epitope-centric view.
The network plots above provide an alternate view of the data, where chords represent the blocking relationships, envelopes inscribe the bins, and colors depict merged data.
A binning dendrogram, showing an adjustable custom cut-height (red line at 4.1) next to a community plot that is clustered by the custom cut height, which provides a less granular view than the four network plots above where the cut height was zero.