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…a competition assay of all the antibodies versus each other in this checkerboard format. And here, red means that there is competition and green means there is not. And so,…
…cell, self-block. 0:20:18.8 TY: So if we zoom in on the lower portion of this heat map, looking at communities that we assigned as five and community 6 and community…
…epitope specificity is an innate property that cannot be predicted. The epitope is highly complex and unknown a priori, and the in silico design of an equally complex complementary paratope…
…left and PTM2 on the right, comprise this combination of PTMs. We call one proximal and one distal. What we did was generate recombinant antibody candidates to the combination of…
…of the particular binding domains in the community plots we have purple communities and a blue community indicated on the structure. So again, really early on with not a lot…
…very similar, competing with each other, competing with basically all the same… And at this level of stringency on the dendrogram, we’re effectively able to communicate confidently that yes, they…
…the frequency also highly comparable of the two groups. 0:33:44.0 DL: And we also did comparison in terms of the identity. So that shows percentage of to the germline. So…
…repertoire, combining VDJ recombination from humanity with somatic hyper-mutation from humanity on the specific frameworks. We then combined that stuff using entirely synthetics framework. So I didn’t want any mutations…
…to find some really discrete, shared behaviors to make some nice communities. I’ll highlight a couple of those here. 0:44:03.5 DB: This is a community that was identified, you can…
…because it actually relates back to the community data I just showed in the sense that the highest affinity clones that actually competed the best in this competition assay with…