View our presentation and poster from this year’s Antibody Engineering & Therapeutics conference.
Daniel Bedinger, PhD, our West Coast Senior Field Applications Scientist spoke about accelerating your antibody discovery with high throughput Array SPR.
Carterra’s new LSA™ platform enables high throughput antibody characterization, including kinetics, affinity, epitope binning, mapping, and quantitation with minimal sample consumption. We will demonstrate capture kinetics and epitope binning studies on a 384-antibody array, providing key parameters to make informed decisions quickly and confidently to streamline library-to-leads.
Daniel Bedinger, PhD, our West Coast Senior Field Applications Scientist presented a poster on the advent of array SPR for the rapid generation of kinetic screening data from up to 384 samples in parallel.
Throughput, speed, resolution, and sample consumption are typically key limiting factors for detailed kinetic characterization early in monoclonal antibody (mAb) discovery campaigns. Here, we show that Array SPR can facilitate the generation of high-quality kinetic data from a large panel of clones rapidly and with minimal sample consumption. In this example of a single day’s run, 384 independent kinetic measurements were made on an array comprised of 43 unique mAbs, each immobilized at 8-16 capacities, using a capture approach which does not require purified antibodies. This method required <1 μg per mAb and only 2 μg of the recombinant monomeric antigen. The array format provided well-described and highly reproducible kinetic measurements for clones spanning a 10,000-fold affinity range for their target antigen. These data clearly demonstrate the efficiency and quality of kinetic analysis that is possible using Array SPR.
Sarah Nelson and Christian Giddens from Carterra enjoyed talking to the people who visited our booth in the exhibit hall.